Diagnostic techniques to detect cryptic leishmaniasis in dogs.
نویسندگان
چکیده
This study of several techniques for detecting cryptic leishmaniasis in dogs from areas in Spain where Leishmania infantum is highly endemic concludes that immunological techniques (enzyme-linked immunosorbent assay, immunofluorescence antibody test, Western blotting, delayed-type hypersensitivity reaction, and in vitro lymphocyte proliferation assay) do not clearly differentiate between noninfected and infected asymptomatic dogs and that culture and PCR are more reliable diagnostic tools.
منابع مشابه
A Molecular and Serological Study on Visceral Leishmaniasis in Asymptomatic Stray Dogs in Mashhad, Iran
Zoonotic visceral leishmaniasis is caused by Leishmania infantum (L. infantum),and its major reservoir hosts are domestic dogs, most of which are asymptomatic. This study aimed to detect L. infantum spp. in asymptomatic stray dogs by molecular and serological methods in Mashhad, Iran, during 2011-12. In this study, 94 asymptomatic stray dogs were randomly selected and their blood samples were c...
متن کاملEvaluation of rK39 Rapid Diagnostic Tests for Canine Visceral Leishmaniasis: Longitudinal Study and Meta-Analysis
BACKGROUND There is a need for sensitive and specific rapid diagnostic tests (RDT) for canine visceral leishmaniasis. The aims of this study were to evaluate the diagnostic performance of immunochromatographic dipstick RDTs using rK39 antigen for canine visceral leishmaniasis by (i) investigating the sensitivity of RDTs to detect infection, disease and infectiousness in a longitudinal cohort st...
متن کاملInfectiousness in a cohort of brazilian dogs: why culling fails to control visceral leishmaniasis in areas of high transmission.
The elimination of seropositive dogs in Brazil has been used to control zoonotic visceral leishmaniasis but with little success. To elucidate the reasons for this, the infectiousness of 50 sentinel dogs exposed to natural Leishmania chagasi infection was assessed through time by xenodiagnosis with the sandfly vector, Lutzomyia longipalpis. Eighteen (43%) of 42 infected dogs became infectious af...
متن کامل[Canine Visceral Leishmaniasis: diagnostic tests do not detect real state of the infection].
OBJECTIVES Establishing indirect immunofluorescence (IFAT) and enzyme-inked immunoassay (ELISA) serological tests' ability to detect canine visceral leishmaniasis (CVL) infection. METHODS 211 cross-bred dogs from the south of the Tolima department (a visceral leishmaniasis endemic area) were evaluated by clinical and serological exams, using the Colombian Leishmania infantum (infantum) MHOM/C...
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Visceral leishmaniasis is a parasitic infectious disease from dogs and canids to human that is caused by protozoans of the genus Leishmania. Information on the prevalence of canine leishmaniasis is necessary to define control measures for zoonotic leishmaniasis. This seroepidemiological survey was performed in dogs from Ahvaz district using by DAT and ELISA. Blood was randomly collected in 38 p...
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ورودعنوان ژورنال:
- Clinical and diagnostic laboratory immunology
دوره 9 5 شماره
صفحات -
تاریخ انتشار 2002